How Does 13C UBT System Work?

Mechanism Helicobacter pylori (H. pylori) produces significant amounts of an enzyme called urease. When you ingest a solution or tablet containing a special non-radioactive carbon marker, this enzyme breaks it down. The process releases 13C-labeled carbon dioxide, which is then absorbed into your bloodstream and exhaled in your breath.

Procedure

You provide an initial breath sample to establish your baseline level. 

 You then drink a solution (or swallow a tablet) containing the 13C-labeled urea. 

fter a waiting period of about 15-30 minutes, you provide a second breath sample.

Measurement 

The testing system compares the amount of 13CO₂ in your two breath samples. It calculates the change, known as the Delta Over Baseline (DOB). A significant increase indicates the presence of active H. pylori infection.

Detection Technology

Specialized analyzers, often using highly accurate techniques like infrared spectroscopy, detect and measure the precise ratio of 13CO₂ in your breath samples.

Features

Kit Contents 

A standard test kit typically includes the13C-urea in tablet or powder form, a citric acid solution to aid ingestion, and sterile breath collection bags. 

Analyzer Systems 

Devices such as the Headway HCBT-01 or Exalenz BreathID analyzers are commonly used in clinics to process the samples automatically. 

Key Advantages 

These systems are designed for efficiency and accuracy, often featuring automated batch testing, high sensitivity and specificity, and the delivery of rapid results.

Applications 

Initial Diagnosis

The primary use is to accurately detect an active H. pylori infection. 

Post-Treatment 

Confirmation It is the standard method to verify successful eradication of the bacteria after a course of antibiotic therapy.

Safety Profile

The 13C isotope used in this test is completely non-radioactive and is considered safe for all patients, including children and pregnant women.